3ON0
Crystal structure of the pED208 TraM-sbmA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1158 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 93.025, 154.687, 167.565 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 83.780 - 2.874 |
| R-factor | 0.25156 |
| Rwork | 0.250 |
| R-free | 0.27811 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g7o C-terminal domain with residues mutated to pED208 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.348 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.789 | 3.000 |
| High resolution limit [Å] | 2.870 | 2.870 |
| Number of reflections | 27538 | |
| <I/σ(I)> | 23 | 3 |
| Completeness [%] | 81.0 | 94.2 |
| Redundancy | 6.3 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 36% MPD, 5% PEG 2000, 100mM cacodylic acid, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
