3OMZ
Crystal structure of MICA-specific human gamma delta T cell receptor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2007-10-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0000 |
Spacegroup name | P 43 |
Unit cell lengths | 112.370, 112.370, 108.110 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.830 - 3.040 |
R-factor | 0.26728 |
Rwork | 0.265 |
R-free | 0.30779 |
Structure solution method | MAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.418 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXS |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.160 |
High resolution limit [Å] | 3.040 | 3.040 |
Rmerge | 0.054 | 0.365 |
Number of reflections | 25563 | |
<I/σ(I)> | 48.4 | 2.1 |
Completeness [%] | 99.8 | 99.1 |
Redundancy | 4.1 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 295 | 0.4M ammonium sulfate, 1.0M lithium sulfate, 0.1M sodium citrate, 0.03M glycyl-glycyl-glycine, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K |