3OMT
Putative antitoxin component, CHU_2935 protein, from Xre family from Prevotella buccae.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.855, 44.882, 86.408 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.800 - 1.650 |
| R-factor | 0.1384 |
| Rwork | 0.136 |
| R-free | 0.19190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bs3 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.432 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 39.800 | 40.000 | 1.680 |
| High resolution limit [Å] | 1.650 | 4.480 | 1.650 |
| Rmerge | 0.098 | 0.034 | 0.895 |
| Number of reflections | 19302 | ||
| <I/σ(I)> | 7.1 | 2.74 | |
| Completeness [%] | 100.0 | 99.9 | 100 |
| Redundancy | 11.5 | 10.8 | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 277 | 2 M ammonium sulfate, 0.1 M phosphate citrate-buffer, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
