3OMC

Structure of human SND1 extended tudor domain in complex with the symmetrically dimethylated arginine PIWIL1 peptide R4me2s

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2010-06-11
Detector	RIGAKU RAXIS HTC
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	35.622, 75.834, 80.177
Unit cell angles	90.00, 90.37, 90.00

Refinement procedure

Resolution	25.050 - 1.770
R-factor	0.1803
Rwork	0.179
R-free	0.21310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2o4x
RMSD bond length	0.010
RMSD bond angle	1.175
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (refmac_5.6.0081)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.830
High resolution limit [Å]	1.770	3.810	1.770
Rmerge	0.040	0.018	0.282
Number of reflections	41508
<I/σ(I)>	18.8
Completeness [%]	99.6	99.6	98
Redundancy	3.6	3.7	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	30% PEG1500, 1:500 V8 PROTEASE, PH 7.5, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 291K