3OM1
Crystal structure of the GluK5 (KA2) ATD dimer at 1.7 Angstrom Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-09-22 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 1.00000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 109.344, 65.635, 113.772 |
Unit cell angles | 90.00, 95.72, 90.00 |
Refinement procedure
Resolution | 29.894 - 1.677 |
R-factor | 0.1696 |
Rwork | 0.168 |
R-free | 0.19630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3om0 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.077 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6.2_432)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.740 |
High resolution limit [Å] | 1.677 | 1.680 |
Rmerge | 0.065 | 0.401 |
Number of reflections | 91506 | |
<I/σ(I)> | 18.1 | 2.4 |
Completeness [%] | 96.6 | 77.3 |
Redundancy | 3.9 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 293 | 0.1 M BICINE, 20% PEG 8K, pH 8.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |