3OM0
Crystal structure of the GluK5 (KA2) ATD crystallographic dimer at 1.4 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-22 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.00000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 66.314, 101.987, 116.225 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.885 - 1.401 |
| R-factor | 0.1761 |
| Rwork | 0.175 |
| R-free | 0.19610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3om0 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.594 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.043 | 0.645 |
| Number of reflections | 77538 | |
| <I/σ(I)> | 29.21 | 2.5 |
| Completeness [%] | 98.6 | 96.4 |
| Redundancy | 5.7 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 293 | 0.1 M CHES, 20% PEG 6K, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
