3OLO
Crystal structure of a PAS domain from two-component sensor histidine kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-30 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 85.430, 85.430, 67.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.202 - 2.094 |
| R-factor | 0.1799 |
| Rwork | 0.177 |
| R-free | 0.22680 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.000 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.140 |
| High resolution limit [Å] | 2.094 | 2.100 |
| Rmerge | 0.138 | 0.743 |
| Number of reflections | 15260 | |
| <I/σ(I)> | 16.6 | 2.4 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 6.6 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 297 | 0.05 M citric acid, 0.05 M BIS-TRIS propane, 16% (w/v) PEG3350, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
