3OLN
Crystal structure of the SRA domain of E3 ubiquitin-protein ligase UHRF2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-08-03 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.528, 46.259, 58.307 |
| Unit cell angles | 104.55, 93.87, 90.08 |
Refinement procedure
| Resolution | 19.730 - 2.300 |
| R-factor | 0.18682 |
| Rwork | 0.184 |
| R-free | 0.23387 |
| Structure solution method | MR |
| Starting model (for MR) | 3clz |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.079 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 16102 | |
| <I/σ(I)> | 24.5256 | 3.73 |
| Completeness [%] | 93.0 | 68.4 |
| Redundancy | 3.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 291 | 23% PEG3350, 0.1 M HEPES-Na, 5% MPD, 0.2 M NaCl, 1 mM DTT, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
