3OL4
Crystal structure of a putative uncharacterized protein from Mycobacterium smegmatis, an ortholog of Rv0543c
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-08-11 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 71.210, 72.690, 45.130 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.000 |
R-factor | 0.204 |
Rwork | 0.202 |
R-free | 0.24220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ol3 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.237 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 8.940 | 2.050 |
High resolution limit [Å] | 2.000 | 6.320 | 2.000 |
Rmerge | 0.048 | 0.019 | 0.285 |
Number of reflections | 16088 | 362 | 1041 |
<I/σ(I)> | 19.25 | 50.4 | 3.3 |
Completeness [%] | 98.0 | 96.8 | 86.5 |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 52.57 mg/mL MysmA,17112.a.A1 PS00688 against PACT screen condition F12, 0.2 M sodium malonate, 0.1 M BisTris Propane pH 6.5, 20% PEG 3350 with 25% ethylene glycol as cryo-protectant, crystal tracking ID 217010f12, VAPOR DIFFUSION, SITTING DROP, temperature 289K |