3OKZ
Crystal Structure of protein gbs0355 from Streptococcus agalactiae, Northeast Structural Genomics Consortium Target SaR127
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 99.049, 41.392, 161.506 |
| Unit cell angles | 90.00, 103.44, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.24600 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.235 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.700 |
| Rmerge | 0.099 |
| Number of reflections | 17016 |
| <I/σ(I)> | 12.3 |
| Completeness [%] | 96.0 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microbatch under oil | 5 | 277 | Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5) . Reservoir solution: NH4SCN 0.1M, Na-acetate 0.1M, PEG 400 20%, microbatch under oil, temperature 277K |
