3OKQ
Crystal structure of a core domain of yeast actin nucleation cofactor Bud6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 58.883, 95.214, 70.787 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.394 - 2.044 |
| R-factor | 0.1946 |
| Rwork | 0.192 |
| R-free | 0.22650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3onx |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.881 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.110 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.064 | 0.276 |
| Number of reflections | 12868 | |
| <I/σ(I)> | 17.6 | 4.1 |
| Completeness [%] | 99.4 | 97.2 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 12% PEG 3350, 175 mM sodium malonate (pH 7.0), 20% glycerol, 5 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
