3OKC
Crystal structure of Corynebacterium glutamicum PimB' bound to GDP (orthorhombic crystal form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.852, 86.400, 106.639 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.360 - 2.400 |
| R-factor | 0.19245 |
| Rwork | 0.190 |
| R-free | 0.23617 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3oka |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.090 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.370 | 2.350 |
| High resolution limit [Å] | 2.230 | 2.230 |
| Rmerge | 0.093 | 0.382 |
| Number of reflections | 19982 | |
| <I/σ(I)> | 9.9 | 2.3 |
| Completeness [%] | 95.8 | 72.5 |
| Redundancy | 3.2 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.1 | 291 | 0.1 M sodium citrate pH 5.1, 22% PEG 3350 , VAPOR DIFFUSION, temperature 291K |
