3OKA
Crystal structure of Corynebacterium glutamicum PimB' in complex with GDP-Man (triclinic crystal form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-09-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9762 |
Spacegroup name | P 1 |
Unit cell lengths | 44.070, 50.020, 85.300 |
Unit cell angles | 92.05, 92.68, 89.94 |
Refinement procedure
Resolution | 43.800 - 2.200 |
R-factor | 0.19097 |
Rwork | 0.189 |
R-free | 0.22100 |
Structure solution method | MIR |
RMSD bond length | 0.008 |
RMSD bond angle | 1.150 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.800 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.073 | 0.238 |
Number of reflections | 35191 | |
<I/σ(I)> | 11.6 | 5.9 |
Completeness [%] | 95.1 | 81.2 |
Redundancy | 2.9 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.5 | 291 | 6-26% PEG 3350, 0.1 M Bis-Tris pH 5.5, 0.1 M lithium sulfate and 0.1 M glycine, VAPOR DIFFUSION, temperature 291K |