3OK8
I-BAR OF PinkBAR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-BM |
Synchrotron site | APS |
Beamline | 17-BM |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2009-07-24 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 99.920, 39.913, 120.702 |
Unit cell angles | 90.00, 94.82, 90.00 |
Refinement procedure
Resolution | 37.050 - 2.250 |
R-factor | 0.225 |
Rwork | 0.221 |
R-free | 0.27900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.957 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CCP4 (MOLREP) |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.330 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.340 | |
Number of reflections | 21987 | |
<I/σ(I)> | 24.1 | 3 |
Completeness [%] | 95.6 | 80 |
Redundancy | 11.8 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 20MM HEPES, 250MM NACL, 5% GLYCEROL, PH 7.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K |