3OK4

Crystal structure of the ANA:RNA decamer suffering from lattice translocation defects

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	CCD
Collection date	2008-09-29
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	34.220, 34.210, 170.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	85.460 - 1.149
R-factor	0.22295
Rwork	0.221
R-free	0.25288
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	2.060
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELX (D/E)
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	85.460	85.460	1.210
High resolution limit [Å]	1.149	3.640	1.149
Number of reflections	70276
<I/σ(I)>	13.6
Completeness [%]	97.2	93.1	95.3
Redundancy	8.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	ammonium sulphate, VAPOR DIFFUSION, HANGING DROP, temperature 289K