3OJW

Disulfide crosslinked cytochrome P450 reductase inactive

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-10-26
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	65.803, 72.958, 138.208
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.660 - 2.200
R-factor	0.205
Rwork	0.205
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1amo
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CNS (1.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.200
Number of reflections	33491
<I/σ(I)>	27.5
Completeness [%]	97.0
Redundancy	8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	292	100 mM HEPES, pH 7.2, 150 mM MgCl2 and 17% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 292KK