3OJA

Crystal structure of LRIM1/APL1C complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2009-08-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9395
Spacegroup name	P 21 21 21
Unit cell lengths	109.890, 110.890, 168.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.560 - 2.700
R-factor	0.2082
Rwork	0.205
R-free	0.26510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.347
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.1.4)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.770
High resolution limit [Å]	2.700	12.070	2.700
Rmerge	0.149	0.063	0.715
Number of reflections	56906	689	4170
<I/σ(I)>	7.44	16.5	2.1
Completeness [%]	99.4	92.2	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	277	15% PEG 1000, 0.1M NaCl, 50 mM Na-Hepes, pH 7.5, vapor diffusion, temperature 277K