3OJ5
Mycobacterium tuberculosis ferritin homolog, BfrB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 231.768, 231.997, 114.324 |
| Unit cell angles | 90.00, 94.67, 90.00 |
Refinement procedure
| Resolution | 49.899 - 2.845 |
| R-factor | 0.2091 |
| Rwork | 0.207 |
| R-free | 0.23950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.198 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX ((phenix.refine: 1.6.2_432)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 49.899 |
| High resolution limit [Å] | 2.845 |
| Number of reflections | 139618 |
| Completeness [%] | 98.9 |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 277 | 14% (w/v) PEG 3350, 5% (w/v) Tacsimate, 100 mM glycine, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
