3OJ2
Crystal structure of FGF1 complexed with the ectodomain of FGFR2b harboring the A172F Pfeiffer syndrome mutation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 173 |
Detector technology | CCD |
Collection date | 2002-07-02 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.277 |
Spacegroup name | P 32 |
Unit cell lengths | 77.945, 77.945, 137.555 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.000 - 2.200 |
Rwork | 0.262 |
R-free | 0.29400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1nun |
RMSD bond length | 0.008 |
RMSD bond angle | 1.420 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.280 |
High resolution limit [Å] | 2.200 | 4.730 | 2.200 |
Number of reflections | 47189 | ||
<I/σ(I)> | 25.4 | 50.9 | 5.9 |
Completeness [%] | 99.3 | 94.1 | 99.6 |
Redundancy | 3.5 | 3.3 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M Hepes, 20% PEG4000, 0.2M ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |