3OJ2

Crystal structure of FGF1 complexed with the ectodomain of FGFR2b harboring the A172F Pfeiffer syndrome mutation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	173
Detector technology	CCD
Collection date	2002-07-02
Detector	ADSC QUANTUM 4
Wavelength(s)	1.277
Spacegroup name	P 32
Unit cell lengths	77.945, 77.945, 137.555
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.000 - 2.200
Rwork	0.262
R-free	0.29400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nun
RMSD bond length	0.008
RMSD bond angle	1.420
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.280
High resolution limit [Å]	2.200	4.730	2.200
Number of reflections	47189
<I/σ(I)>	25.4	50.9	5.9
Completeness [%]	99.3	94.1	99.6
Redundancy	3.5	3.3	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.1M Hepes, 20% PEG4000, 0.2M ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K