3OIW
H-RasG12V with allosteric switch in the "on" state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | H 3 2 |
Unit cell lengths | 88.519, 88.519, 134.385 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 26.606 - 1.300 |
R-factor | 0.1974 |
Rwork | 0.196 |
R-free | 0.21020 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.132 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.6.1_336) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.240 |
High resolution limit [Å] | 1.200 | 2.590 | 1.200 |
Rmerge | 0.056 | 0.041 | |
Number of reflections | 62549 | ||
<I/σ(I)> | 13.3 | ||
Completeness [%] | 98.7 | 99.5 | 87.7 |
Redundancy | 9.5 | 10.4 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | sitting drop | 7.5 | 298 | 200 Mm Calcium Acetate, 20 % PEG 3350, pH 7.5, sitting drop, temperature 298K |