3OIV
H-RasG12V with allosteric switch in the "off" state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | H 3 2 |
Unit cell lengths | 87.883, 87.883, 133.057 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 26.404 - 1.837 |
R-factor | 0.1621 |
Rwork | 0.158 |
R-free | 0.19780 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.221 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.6.1_336) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.910 |
High resolution limit [Å] | 1.840 | 3.960 | 1.840 |
Rmerge | 0.131 | 0.049 | 0.643 |
Number of reflections | 17438 | ||
<I/σ(I)> | 5.6 | ||
Completeness [%] | 99.7 | 99.6 | 97.7 |
Redundancy | 10.1 | 10.4 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | sitting drop | 7.5 | 298 | 200 Mm Calcium Chloride, 20 % PEG 3350, pH 7.5, sitting drop, temperature 298K |