3OIO
Crystal structure of transcriptional regulator (AraC-type DNA-binding domain-containing proteins) from Chromobacterium violaceum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 65 |
| Unit cell lengths | 96.753, 96.753, 33.312 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.670 - 1.650 |
| R-factor | 0.1627 |
| Rwork | 0.161 |
| R-free | 0.18190 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.473 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.670 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.070 | 0.544 |
| Number of reflections | 21721 | |
| <I/σ(I)> | 49.3 | 5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11 | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 297 | 0.1M Bis-Tris propane, 1.5M Ammonium Sulfate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
