3OIC
Crystal Structure of Enoyl-ACP Reductases III (FabL) from B. subtilis (apo form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97787 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 85.211, 93.426, 66.324 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.710 - 2.200 |
| R-factor | 0.22845 |
| Rwork | 0.226 |
| R-free | 0.28179 |
| Structure solution method | SAD |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.034 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.087 | 0.732 |
| Total number of observations | 560220 | |
| Number of reflections | 27593 | |
| <I/σ(I)> | 49 | 3.07 |
| Completeness [%] | 99.8 | 98.3 |
| Redundancy | 5.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 277 | 0.2M MgCl2, 0.1M imidazole, 15% Ethanol, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
