3OI8
The crystal structure of functionally unknown conserved protein domain from Neisseria meningitidis MC58
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97926, 0.97942 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.640, 52.156, 64.010 |
| Unit cell angles | 90.00, 112.67, 90.00 |
Refinement procedure
| Resolution | 39.095 - 1.989 |
| R-factor | 0.185 |
| Rwork | 0.182 |
| R-free | 0.23200 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.050 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.100 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.134 | 0.520 |
| Number of reflections | 22323 | |
| <I/σ(I)> | 16.2 | 1.07 |
| Completeness [%] | 98.4 | 72 |
| Redundancy | 4.7 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 20% w/v PEG8000, 0.1M Tris, 0.2M MgCl2, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
