3OHP
Crystal structure of HGPRT from Vibrio cholerae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-03 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0750 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.511, 145.213, 51.611 |
Unit cell angles | 90.00, 94.76, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.040 |
R-factor | 0.18235 |
Rwork | 0.180 |
R-free | 0.22421 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g9s |
RMSD bond length | 0.013 |
RMSD bond angle | 1.444 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP (10.2.23) |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.110 |
High resolution limit [Å] | 2.040 | 2.040 |
Rmerge | 0.070 | 0.353 |
Number of reflections | 46597 | |
<I/σ(I)> | 17.8 | 3.4 |
Completeness [%] | 99.1 | 92.6 |
Redundancy | 4 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1M citrate PH 5.5, 20% PEG3000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |