3OGV
Complex structure of beta-galactosidase from Trichoderma reesei with PETG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 1 |
| Unit cell lengths | 67.600, 68.700, 81.700 |
| Unit cell angles | 108.50, 97.70, 114.50 |
Refinement procedure
| Resolution | 42.760 - 1.400 |
| R-factor | 0.13388 |
| Rwork | 0.132 |
| R-free | 0.16580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3og2 |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.467 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.500 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 220582 | |
| Completeness [%] | 92.6 | 92.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 8% PEG 8000, 0.1M sodium cacodylate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
