3OGR
Complex structure of beta-galactosidase from Trichoderma reesei with galactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 |
| Unit cell lengths | 67.430, 69.280, 81.500 |
| Unit cell angles | 109.03, 97.34, 114.38 |
Refinement procedure
| Resolution | 19.780 - 1.500 |
| R-factor | 0.1303 |
| Rwork | 0.128 |
| R-free | 0.16758 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3og2 |
| RMSD bond length | 0.025 |
| RMSD bond angle | 1.977 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.600 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 179614 | |
| Completeness [%] | 92.6 | 92.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 8% PEG 8000, 0.1M sodium cacodylate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
