3OGM
Structure of COI1-ASK1 in complex with coronatine and the JAZ1 degron
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 103 |
Detector technology | CCD |
Collection date | 2008-07-26 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 123.170, 220.758, 149.540 |
Unit cell angles | 90.00, 104.45, 90.00 |
Refinement procedure
Resolution | 49.942 - 3.340 |
R-factor | 0.2266 |
Rwork | 0.226 |
R-free | 0.26980 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.274 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.410 |
High resolution limit [Å] | 3.340 | 3.340 |
Number of reflections | 95997 | |
Completeness [%] | 92.9 | 94.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 278 | 1.7M ammonium phosphate, 100mM BTP, 100mM sodium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 278K |