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3OGK

Structure of COI1-ASK1 in complex with coronatine and an incomplete JAZ1 degron

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsALS BEAMLINE 8.2.1
Synchrotron siteALS
Beamline8.2.1
Temperature [K]103
Detector technologyCCD
Collection date2008-06-19
DetectorADSC QUANTUM 315
Wavelength(s)1.0
Spacegroup nameP 1 21 1
Unit cell lengths121.847, 221.455, 148.474
Unit cell angles90.00, 104.49, 90.00
Refinement procedure
Resolution49.629 - 2.800
R-factor0.2267
Rwork0.225
R-free0.26820
RMSD bond length0.014
RMSD bond angle1.570
Data reduction softwareDENZO
Data scaling softwareHKL-2000
Phasing softwarePHENIX
Refinement softwarePHENIX ((phenix.refine))
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.900
High resolution limit [Å]2.8002.800
Number of reflections174966
Completeness [%]100.0100
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP72781.7M ammonium phosphate, 100mM BTP, 100mM sodium chloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 278K

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