3OGK
Structure of COI1-ASK1 in complex with coronatine and an incomplete JAZ1 degron
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2008-06-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 121.847, 221.455, 148.474 |
| Unit cell angles | 90.00, 104.49, 90.00 |
Refinement procedure
| Resolution | 49.629 - 2.800 |
| R-factor | 0.2267 |
| Rwork | 0.225 |
| R-free | 0.26820 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.570 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 174966 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 278 | 1.7M ammonium phosphate, 100mM BTP, 100mM sodium chloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
