3OGH
Crystal structure of yciE protein from E. coli CFT073, a member of ferritine-like superfamily of diiron-containing four-helix-bundle proteins
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9794 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 30.148, 90.295, 58.132 |
Unit cell angles | 90.00, 103.51, 90.00 |
Refinement procedure
Resolution | 40.000 - 1.650 |
R-factor | 0.16878 |
Rwork | 0.166 |
R-free | 0.21291 |
Structure solution method | SAD |
RMSD bond length | 0.022 |
RMSD bond angle | 1.794 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.680 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.083 | 0.367 |
Number of reflections | 36634 | |
<I/σ(I)> | 29 | 4 |
Completeness [%] | 99.8 | 99.5 |
Redundancy | 3.7 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 25% Peg 3350, 0.2 M Magnesium Chloride, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |