3OGC
KcsA E71A variant in presence of Na+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2010-04-24 |
Detector | ADSC Q315 |
Wavelength(s) | 1.100 |
Spacegroup name | I 4 |
Unit cell lengths | 154.735, 154.735, 75.401 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.700 - 3.800 |
R-factor | 0.24872 |
Rwork | 0.247 |
R-free | 0.28289 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2atk |
RMSD bond length | 0.005 |
RMSD bond angle | 0.791 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 38.700 | 50.000 | 3.890 |
High resolution limit [Å] | 3.800 | 9.350 | 3.800 |
Rmerge | 0.107 | 0.024 | 0.445 |
Number of reflections | 8872 | ||
<I/σ(I)> | 7.1 | ||
Completeness [%] | 99.8 | 98.2 | 100 |
Redundancy | 3.6 | 3.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG 400, MgAcetate, MES, pH 6.5, hanging drop, temperature 293K, VAPOR DIFFUSION, HANGING DROP |