3OG2

Native crystal structure of Trichoderma reesei beta-galactosidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2008-04-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97926
Spacegroup name	P 1
Unit cell lengths	67.300, 69.100, 81.500
Unit cell angles	109.10, 97.30, 114.50

Refinement procedure

Resolution	20.000 - 1.200
R-factor	0.1392
Rwork	0.139
R-free	0.17090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1tg7
RMSD bond length	0.014
RMSD bond angle	0.030
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.300
High resolution limit [Å]	1.200	1.200
Number of reflections	341073
Completeness [%]	90.6	90.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	8% PEG 8000, 0.1M sodium cacodylate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K