3OFM
Structure of a human CK2alpha prime, the paralog isoform of the catalytic subunit of protein kinase CK2 from Homo sapiens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-06 |
Detector | MARRESEARCH |
Wavelength(s) | 0.91841 |
Spacegroup name | P 1 |
Unit cell lengths | 46.690, 47.570, 50.570 |
Unit cell angles | 112.40, 89.49, 91.30 |
Refinement procedure
Resolution | 33.030 - 2.000 |
R-factor | 0.17378 |
Rwork | 0.172 |
R-free | 0.20721 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2pvr |
RMSD bond length | 0.007 |
RMSD bond angle | 0.975 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.030 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.057 | 0.257 |
Number of reflections | 23663 | |
<I/σ(I)> | 12.61 | 3.4 |
Completeness [%] | 86.8 | 47.7 |
Redundancy | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | Protein stock solution: 6 mg/ml protein, 25 mM Tris/HCl, pH 8.5, 500 mM sodium chloride, 1 mM inhibitor 3-(4,5,6,7-tetrabromo-1H-benzotriazol-1-yl)propan-1-ol Reservoir: 28 % PEG6000, 500 mM lithium chloride, 100 mM Tris/HCl, pH 8.5 Drop: mixture of 0.5 uL protein solution plus 0.5 uL reservoir, VAPOR DIFFUSION, SITTING DROP, temperature 293K |