3OFH

Structured Domain of Mus musculus Mesd

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4C
Synchrotron site	NSLS
Beamline	X4C
Temperature [K]	100
Detector technology	CCD
Collection date	2007-04-24
Detector	MAR CCD 165 mm
Wavelength(s)	0.9790
Spacegroup name	P 43
Unit cell lengths	71.651, 71.651, 37.102
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.934 - 2.013
R-factor	0.1502
Rwork	0.147
R-free	0.17440
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.563
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (1.3.1)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.080
High resolution limit [Å]	2.010	4.330	2.010
Rmerge	0.038	0.023	0.148
Number of reflections	12634
<I/σ(I)>	23.1
Completeness [%]	99.0	96.3	96.4
Redundancy	3.8	3.5	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	0.7-0.9M AmPO4 and 100mM Hepes pH7.5, streak seeded with micro-crystals, vapor diffusion, hanging drop, temperature 277K