3OFF

Structured Domain of Drosophila melanogaster Boca p65 2 2 Crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4C
Synchrotron site	NSLS
Beamline	X4C
Temperature [K]	100
Detector technology	CCD
Collection date	2009-03-09
Detector	MAR CCD 165 mm
Wavelength(s)	0.9790
Spacegroup name	P 65 2 2
Unit cell lengths	62.284, 62.284, 89.675
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.419 - 2.002
R-factor	0.1935
Rwork	0.190
R-free	0.22570
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.667
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.13)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.030
High resolution limit [Å]	2.000	5.420	2.000
Rmerge	0.129	0.064	0.430
Number of reflections	13036
<I/σ(I)>	7.1
Completeness [%]	99.0	92.1	100
Redundancy	11.1	10.3	9.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.7	293	48-52% saturated AmSO4, 100mM NaAc pH 5.6-5.8 at 298 C, one of four crystal morphologies, vapor diffusion, hanging drop, temperature 293K, VAPOR DIFFUSION, HANGING DROP