3OFE

Structured Domain of Drosophila melanogaster Boca p41 2 2 Crystal form

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2005-09-14
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9795 0.9802 0.9645
Spacegroup name	P 41 2 2
Unit cell lengths	34.106, 34.106, 311.238
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.781 - 2.288
R-factor	0.1911
Rwork	0.189
R-free	0.22790
Structure solution method	MAD
RMSD bond length	0.003
RMSD bond angle	0.601
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.08)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	2.380
High resolution limit [Å]	2.300	4.930	2.300
Rmerge	0.069	0.035	0.282
Number of reflections	15757
<I/σ(I)>	10
Completeness [%]	99.3	97.6	99.3
Redundancy	6.5	6.1	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	277	18-21% PEG 3350, 100-200mM Na citrate, 100mM CHES pH9.0, and 10mM DTT with macroseeding, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	9	277	18-21% PEG 3350, 100-200mM Na citrate, 100mM CHES pH9.0, and 10mM DTT with macroseeding, VAPOR DIFFUSION, HANGING DROP, temperature 277K