3OF7
The Crystal Structure of Prp20p from Saccharomyces cerevisiae and Its Binding Properties to Gsp1p and Histones
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-16 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9793, 0.9794, 0.9300 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 103.510, 75.131, 92.019 |
Unit cell angles | 90.00, 119.64, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.900 |
R-factor | 0.1908 |
Rwork | 0.189 |
R-free | 0.22420 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.125 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (refmac_5.5.0102) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 79.980 | 50.000 | 1.630 |
High resolution limit [Å] | 1.600 | 4.340 | 1.600 |
Rmerge | 0.079 | 0.045 | 0.507 |
Number of reflections | 71254 | ||
<I/σ(I)> | 11 | ||
Completeness [%] | 87.6 | 97.8 | 35.7 |
Redundancy | 6.9 | 6.7 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 283 | 0.2M tri-Sodium Citrate dihydrate, 15% (w/v) PEG 3350, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 283K |