3OF5
Crystal Structure of a Dethiobiotin Synthetase from Francisella tularensis subsp. tularensis SCHU S4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-12 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9755 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.278, 93.177, 62.732 |
| Unit cell angles | 90.00, 95.78, 90.00 |
Refinement procedure
| Resolution | 26.220 - 1.520 |
| R-factor | 0.1706 |
| Rwork | 0.169 |
| R-free | 0.19780 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.050 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.550 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.047 | 0.310 |
| Number of reflections | 136170 | |
| <I/σ(I)> | 33.8 | 4.77 |
| Completeness [%] | 100 | |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | PEG8K 18%, CaAcetate 0.2M, NaCacod. 0.1M pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
