3OES
Crystal structure of the small GTPase RhebL1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2010-08-10 |
Detector | RIGAKU RAXIS |
Wavelength(s) | 1.5418 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 72.665, 126.745, 41.356 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.301 |
R-factor | 0.215 |
Rwork | 0.212 |
R-free | 0.27100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xtr |
RMSD bond length | 0.015 |
RMSD bond angle | 1.399 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.340 |
High resolution limit [Å] | 2.300 | 6.240 | 2.300 |
Rmerge | 0.105 | 0.040 | 0.850 |
Number of reflections | 8829 | ||
<I/σ(I)> | 8.6 | ||
Completeness [%] | 99.9 | 98.4 | 100 |
Redundancy | 7 | 6.2 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 9.5 | 291 | 1.8M sodium formate, 0.1M lysine buffer, pH 9.5, vapor diffusion, temperature 291K |