3OEO
The crystal structure E. coli Spy
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.11587, 0.97944, 0.97976, 0.98771, 0.97220 |
| Spacegroup name | P 32 |
| Unit cell lengths | 69.195, 69.195, 126.347 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| R-factor | 0.25462 |
| Rwork | 0.252 |
| R-free | 0.29973 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.116 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 5.770 | 2.700 |
| Rmerge | 0.054 | 0.030 | 0.385 |
| Number of reflections | 18354 | ||
| <I/σ(I)> | 15.1 | ||
| Completeness [%] | 98.3 | 99.6 | 95.3 |
| Redundancy | 4.1 | 5.4 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 4.6 | 295 | 0.1 M acetate pH 4.6, 26-32% (w/v) PEG400, 0.2 M CdCl2, and 2 mM TCEP, EVAPORATION, temperature 295K |
