3OE3
Crystal structure of PliC-St, periplasmic lysozyme inhibitor of C-type lysozyme from Salmonella typhimurium
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Wavelength(s) | 0.9793, 0.9755 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 192.383, 34.685, 74.910 |
Unit cell angles | 90.00, 98.41, 90.00 |
Refinement procedure
Resolution | 19.765 - 1.510 |
R-factor | 0.209 |
Rwork | 0.208 |
R-free | 0.24240 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.970 |
Data scaling software | SCALA |
Refinement software | PHENIX (1.5_2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 19.765 | 19.765 | 1.590 |
High resolution limit [Å] | 1.510 | 4.780 | 1.510 |
Rmerge | 0.071 | 0.683 | |
Total number of observations | 10184 | 48573 | |
Number of reflections | 72824 | ||
<I/σ(I)> | 8.3 | 6.4 | 1 |
Completeness [%] | 94.2 | 73.7 | 96.2 |
Redundancy | 5 | 5.3 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 26% (w/v) PEG8000, 0.1M imidazole, 0.2M NaCl, pH 7.5, vapor diffusion, hanging drop, temperature 293K |