3ODM
Archaeal-type phosphoenolpyruvate carboxylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-28 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.03948 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 121.420, 161.580, 279.980 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.840 - 2.950 |
| R-factor | 0.17833 |
| Rwork | 0.178 |
| R-free | 0.22316 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.337 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.830 | 3.030 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.133 | |
| Number of reflections | 116208 | |
| <I/σ(I)> | 17.4 | 2 |
| Completeness [%] | 99.9 | 98.6 |
| Redundancy | 14.3 | 9.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 1.5M sodium malonate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
