3ODJ

Crystal structure of H. influenzae rhomboid GlpG with disordered loop 4, helix 5 and loop 5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Spacegroup name	C 1 2 1
Unit cell lengths	120.927, 35.190, 52.672
Unit cell angles	90.00, 104.06, 90.00

Refinement procedure

Resolution	50.000 - 2.840
R-factor	0.246
Rwork	0.243
R-free	0.29660
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.606
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.950
High resolution limit [Å]	2.840	6.140	2.840
Rmerge	0.069	0.022	0.420
Number of reflections	5066
<I/σ(I)>	11.7
Completeness [%]	96.9	92	98.8
Redundancy	2.1	2	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	vapour diffusion	6	298	PEG 4000, NaCl, Ethanol, glycerol, pH 6.0, vapour diffusion, temperature 298K