3ODB
Haemophilus influenzae ferric binding protein A -Iron Loaded -open Conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 105 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-04-30 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 106.200, 75.577, 33.960 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.620 |
| R-factor | 0.20413 |
| Rwork | 0.202 |
| R-free | 0.25206 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1d9v |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.926 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 106.000 | 1.680 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.053 | 0.310 |
| Number of reflections | 34190 | |
| <I/σ(I)> | 60 | 2.9 |
| Completeness [%] | 96.0 | 70.4 |
| Redundancy | 13.2 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | 25% PEG 1000, 0.1 M MES, pH 6.5 , VAPOR DIFFUSION, temperature 293K |
