3OD7

Haemophilus influenzae ferric binding protein A -Iron Loaded

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	105
Detector technology	IMAGE PLATE
Collection date	2008-04-08
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	130.957, 52.515, 40.989
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.739 - 1.801
R-factor	0.2049
Rwork	0.203
R-free	0.25030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1d9v
RMSD bond length	0.006
RMSD bond angle	0.963
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.6.2_432))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge		0.308
Number of reflections	26076
Completeness [%]	96.5	88.1
Redundancy		3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.6	303	18% PEG 4000, 0.4M Imidazole/Malate, 0.2M NaCl, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 303K