3OD1
The crystal structure of an ATP phosphoribosyltransferase regulatory subunit/histidyl-tRNA synthetase from Bacillus halodurans C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-22 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97967 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.696, 63.255, 107.637 |
| Unit cell angles | 90.00, 105.72, 90.00 |
Refinement procedure
| Resolution | 25.677 - 1.970 |
| R-factor | 0.1815 |
| Rwork | 0.179 |
| R-free | 0.22420 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.020 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.000 | 2.010 |
| High resolution limit [Å] | 1.970 | 1.980 |
| Rmerge | 0.088 | 0.694 |
| Number of reflections | 65055 | |
| <I/σ(I)> | 25.3 | 2 |
| Completeness [%] | 98.5 | 100 |
| Redundancy | 4.1 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.1M HEPES, 12% PEG4000, 0.1M Sodium Acetate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
