3OCO
The crystal structure of a Hemolysin-like protein containing CBS domain of Oenococcus oeni PSU
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97924, 0.97943 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 44.454, 93.556, 92.061 |
| Unit cell angles | 90.00, 102.93, 90.00 |
Refinement procedure
| Resolution | 33.591 - 2.204 |
| R-factor | 0.1995 |
| Rwork | 0.196 |
| R-free | 0.25800 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.224 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.122 | 0.377 |
| Number of reflections | 17816 | |
| <I/σ(I)> | 12.8 | 2.2 |
| Completeness [%] | 96.2 | 77.2 |
| Redundancy | 3.8 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 20% PEG3350, 0.2M LiCl, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
