3OCD
Diheme SoxAX - C236M mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-02-02 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5412 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.496, 66.031, 85.887 |
| Unit cell angles | 112.59, 94.33, 90.02 |
Refinement procedure
| Resolution | 41.180 - 2.250 |
| R-factor | 0.22303 |
| Rwork | 0.221 |
| R-free | 0.25737 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3oa8 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.157 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Number of reflections | 41134 | |
| Completeness [%] | 90.0 | 86.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 25% PEG 3350, 0.2M lithium sulfate, 0.1M Tris-HCl pH 8.5, 0.01M spermidine, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
