3OC5

Crystal Structure of the vibrio cholerae secreted colonization factor TcpF

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-07-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9116,0.9794
Spacegroup name	F 4 3 2
Unit cell lengths	224.880, 224.880, 224.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.650 - 2.400
R-factor	0.23
Rwork	0.229
R-free	0.25600
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.130
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SOLVE
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.400
Number of reflections	19524
Completeness [%]	99.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5	293	(NH4)2 H PO4 1M, Imidazole 100 mM, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K