3OBY
Crystal structure of Archaeoglobus fulgidus Pelota reveals inter-domain structural plasticity
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-03-01 |
Detector | ADSC QUANTUM 4r |
Wavelength(s) | 0.97800 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 136.237, 66.685, 98.380 |
Unit cell angles | 90.00, 107.29, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.900 |
Rwork | 0.209 |
R-free | 0.27900 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.530 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 17950 | |
Completeness [%] | 96.1 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 296 | 100mM imidazole pH 6.0, 200mM MgCl2, 8%(w/v) polyethylene glycol 8000, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 296 | 100mM imidazole pH 6.0, 200mM MgCl2, 8%(w/v) polyethylene glycol 8000, VAPOR DIFFUSION, HANGING DROP, temperature 296K |